7JU3
MtrR bound to the mtrCDE operator from Neisseria gonorrhoeae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.116 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 153.273, 59.420, 69.508 |
| Unit cell angles | 90.00, 114.31, 90.00 |
Refinement procedure
| Resolution | 69.842 - 2.700 |
| R-factor | 0.2329 |
| Rwork | 0.228 |
| R-free | 0.28230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7jnp |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.842 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.071 | 0.440 |
| Rmeas | 0.088 | 0.577 |
| Number of reflections | 15036 | 1809 |
| <I/σ(I)> | 7.7 | 1.8 |
| Completeness [%] | 95.1 | 79.4 |
| Redundancy | 2.7 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 200 mM calcium acetate, 100 mM Tris-HCl pH 7.5, 27% Polyethylene glycol MW-8000 |
