7JTZ
Yeast Glo3 GAP domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-10-22 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.77118 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.369, 74.031, 77.902 |
| Unit cell angles | 90.00, 105.34, 90.00 |
Refinement procedure
| Resolution | 41.200 - 2.070 |
| R-factor | 0.1839 |
| Rwork | 0.181 |
| R-free | 0.23810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p57 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.882 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.202 | 2.140 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmeas | 0.110 | 0.110 |
| Number of reflections | 34082 | 34082 |
| <I/σ(I)> | 13 | |
| Completeness [%] | 93.5 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293.15 | 2.0 M ammonium sulfate, 0.1 M sodium acetate |
