7JTV
Structure of IMPa from Pseudomonas aeruginosa in complex with an O-glycopeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-06 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.340, 156.500, 95.680 |
| Unit cell angles | 90.00, 114.31, 90.00 |
Refinement procedure
| Resolution | 29.960 - 2.450 |
| R-factor | 0.1827 |
| Rwork | 0.180 |
| R-free | 0.23410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kdw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.897 |
| Data reduction software | Aimless |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.140 | 0.686 |
| Rpim | 0.072 | 0.375 |
| Number of reflections | 88358 | 4448 |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 5.2 | 4.8 |
| CC(1/2) | 0.986 | 0.698 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 20% polyethylene glycol 3350, 0.22 M NaH2PO4, 0.1 M HEPES |
