7JTU
Cytidine deaminase T6S toxin from Pseudomonas syringae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2020-02-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9790 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 93.069, 93.069, 383.492 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.390 - 3.000 |
R-factor | 0.2054 |
Rwork | 0.202 |
R-free | 0.23480 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 3.030 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.120 | |
Rpim | 0.279 | |
Number of reflections | 17597 | 434 |
<I/σ(I)> | 22.9 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 28.6 | 29.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Hanging drop diffusion. SsdA/SsdAI complex at 10 mg/mL was mixed 1:1 with crystallization solutions containing 20% PEG 3350, 0.1 M Bis-Tris:HCl pH 7.5 and 200 mM MgCl2. Cryopreservation was in crystallization solution with 20% glycerol. |