7JTN
Human Complement Factor B Inhibited by a Slow Off-Rate Modified Aptamer of 29 Bases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97243 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.126, 144.407, 87.185 |
| Unit cell angles | 90.00, 109.46, 90.00 |
Refinement procedure
| Resolution | 42.900 - 3.100 |
| R-factor | 0.2098 |
| Rwork | 0.208 |
| R-free | 0.24130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ok5 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.621 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.17) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 6.670 | 3.100 |
| Rmerge | 0.226 | 0.127 | 0.864 |
| Rmeas | 0.248 | 0.139 | 0.985 |
| Rpim | 0.101 | 0.054 | 0.461 |
| Total number of observations | 209444 | ||
| Number of reflections | 36509 | 3724 | 3525 |
| <I/σ(I)> | 4.7 | ||
| Completeness [%] | 99.4 | 99.9 | 96 |
| Redundancy | 5.7 | 6.4 | 4.1 |
| CC(1/2) | 0.956 | 0.573 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M bis-tris, 0.2 M ammonium acetate, 25% peg-3350 |
