7JTL

Structure of SARS-CoV-2 ORF8 accessory protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-05-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.000001
Spacegroup name	P 41 21 2
Unit cell lengths	44.258, 44.258, 264.111
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.650 - 2.040
R-factor	0.2214
Rwork	0.217
R-free	0.25740
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.991
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.650	2.110
High resolution limit [Å]	2.040	2.040
Rmerge	0.101	1.592
Rmeas	0.106	1.694
Rpim	0.032	0.555
Number of reflections	17510	1593
<I/σ(I)>	14.92	1.2
Completeness [%]	95.1
Redundancy	10
CC(1/2)	0.999	0.390

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100 mM sodium dihydrogen phosphate pH 6.5, 12 % (w/v) PEG8000