7JTI
Interphotoreceptor retinoid-binding protein (IRBP) in complex with a monoclonal antibody (F3F5 mAb5)
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Energy Filter | GIF Quantum LS |
Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 7.4 |
Number of Particle | 281804 |
Software | PHENIX,UCSF Chimera,SerialEM,cisTEM,cryoSPARC |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |