7JT4
Crystal Structure of BPTF bromodomain labelled with 5-fluoro-tryptophan
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-11-20 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 27.335, 66.763, 39.260 |
Unit cell angles | 90.00, 105.02, 90.00 |
Refinement procedure
Resolution | 19.960 - 2.060 |
R-factor | 0.1664 |
Rwork | 0.165 |
R-free | 0.18620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qzs |
RMSD bond length | 0.002 |
RMSD bond angle | 0.507 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.400 | 2.139 |
High resolution limit [Å] | 2.060 | 2.065 |
Rmerge | 0.033 | 0.090 |
Number of reflections | 7452 | 761 |
<I/σ(I)> | 19.77 | 9.55 |
Completeness [%] | 88.6 | |
Redundancy | 2 | |
CC(1/2) | 0.998 | 0.980 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% (w/v) polyethylene glycol 1500 and 10% (w/v) proprionate-cacodylate-bis tris propane |