7JSB
Self-assembly of a 3D DNA crystal lattice (4x6 scramble duplex version) containing the J30 immobile Holliday junction with R3 symmetry
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 |
| Unit cell lengths | 114.698, 114.698, 50.460 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.396 - 3.103 |
| R-factor | 0.228 |
| Rwork | 0.227 |
| R-free | 0.24150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vy6 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.748 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 8.400 | 3.100 |
| Rmerge | 0.161 | 0.140 | 0.619 |
| Rmeas | 0.170 | 0.148 | 0.660 |
| Rpim | 0.053 | 0.047 | 0.220 |
| Total number of observations | 41400 | ||
| Number of reflections | 4290 | 219 | 182 |
| <I/σ(I)> | 7.6 | ||
| Completeness [%] | 95.8 | 98.6 | 83.9 |
| Redundancy | 9.7 | 10.4 | 6.7 |
| CC(1/2) | 0.963 | 0.916 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mL of 0.05 M Cacodylate pH 6.0 with 10 mM MgCl2, 2.5 mM spermine, 5 mM CaCl2, and 10% isopropanol was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock |
