7JS4
The structure of the M60 catalytic domain with the CBM51-1 and CBM51-2 domains from Clostridium perfringens ZmpB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-002 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-13 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54187 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.908, 119.321, 129.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.690 - 4.600 |
R-factor | 0.1945 |
Rwork | 0.191 |
R-free | 0.25820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kdn |
RMSD bond length | 0.006 |
RMSD bond angle | 1.563 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 4.680 |
High resolution limit [Å] | 4.600 | 4.600 |
Rmerge | 0.204 | 0.741 |
Rpim | 0.092 | 0.348 |
Number of reflections | 6474 | 312 |
<I/σ(I)> | 8.7 | 2.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.8 | 4.8 |
CC(1/2) | 0.977 | 0.849 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 291 | 0.2 M potassium citrate tribasic monohydrate, 20% PEG3350 |