7JRT
Crystal structures of artificially designed homomeric RNA nanoarchitectures
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-12 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.10480 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.436, 71.990, 89.439 |
Unit cell angles | 90.00, 117.61, 90.00 |
Refinement procedure
Resolution | 45.530 - 3.070 |
R-factor | 0.1924 |
Rwork | 0.189 |
R-free | 0.22680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID: 2NOK |
RMSD bond length | 0.006 |
RMSD bond angle | 1.220 |
Data reduction software | HKL-2000 (718) |
Data scaling software | HKL-2000 (718) |
Phasing software | PHENIX (1.10.1_2155) |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.530 | 3.180 |
High resolution limit [Å] | 3.070 | 3.070 |
Rmerge | 0.100 | 0.510 |
Number of reflections | 18129 | 1817 |
<I/σ(I)> | 39.2 | |
Completeness [%] | 99.3 | |
Redundancy | 16.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 80 mM NaCl, 12 mM KCl, 20 mM MgCl2, 40 mM sodium cacodylate pH 6.0, 30 % (v/v) MPD, 12 mM spermine tetrahydrochloride |