7JRS
Crystal structures of artificially designed homomeric RNA nanoarchitectures
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-11 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.979180 |
Spacegroup name | P 1 |
Unit cell lengths | 60.623, 64.795, 69.578 |
Unit cell angles | 85.70, 64.25, 79.91 |
Refinement procedure
Resolution | 38.289 - 3.210 |
R-factor | 0.2768 |
Rwork | 0.275 |
R-free | 0.29660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID: 4CS1 |
RMSD bond length | 0.001 |
RMSD bond angle | 0.318 |
Data reduction software | HKL-2000 (718) |
Data scaling software | HKL-2000 (718) |
Phasing software | PHENIX (1.11.1_2575) |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.270 |
High resolution limit [Å] | 3.210 | 3.210 |
Rmerge | 0.069 | 0.804 |
Number of reflections | 15105 | 790 |
<I/σ(I)> | 29.6 | |
Completeness [%] | 97.8 | 95.9 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 20 mM MgCl2, 0.3 mM spermine tetrahydrochloride, 50 mM sodium succinate pH 5.5, 3.0 M Ammonium Sulfate |