7JRM
The structure of CBM51-2 and INT domains from Clostridium perfringens ZmpB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-11 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91966 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 151.050, 49.160, 78.500 |
Unit cell angles | 90.00, 112.05, 90.00 |
Refinement procedure
Resolution | 41.520 - 2.000 |
R-factor | 0.2493 |
Rwork | 0.248 |
R-free | 0.27170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vmg |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.380 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.109 | 0.352 |
Rpim | 0.060 | 0.206 |
Number of reflections | 36287 | 5210 |
<I/σ(I)> | 7 | 4.2 |
Completeness [%] | 99.6 | 98.9 |
Redundancy | 4.4 | 4.1 |
CC(1/2) | 0.944 | 0.811 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.15 M sodium phosphate monobasic, 18% PEG3350, 0.1 M Tris-HCl |