7JQY

Crystal structure of Cfl1-D123S from Burkholderia cenocepacia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	C 1 2 1
Unit cell lengths	195.000, 98.400, 170.000
Unit cell angles	90.00, 118.60, 90.00

Refinement procedure

Resolution	44.710 - 2.150
R-factor	0.2104
Rwork	0.209
R-free	0.23970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kd2
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.367	2.280
High resolution limit [Å]	2.150	2.150
Number of reflections	152155	23995
<I/σ(I)>	10.96
Completeness [%]	99.3
Redundancy	3.5
CC(1/2)	0.998	0.781

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	6% ethylene glycol, 8% PEG 8000, 100 mM HEPES pH 7.6