7JQH

Structure of wild type Glyoxylate/Hydroxypyruvate reductase A from Escherichia Coli in complex with phosphate and NADP+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 65 2 2
Unit cell lengths	158.768, 158.768, 97.699
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.030 - 2.000
R-factor	0.1536
Rwork	0.153
R-free	0.17370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kbo
RMSD bond length	0.014
RMSD bond angle	1.280
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.030	2.070
High resolution limit [Å]	1.999	1.999
Number of reflections	49378	4836
<I/σ(I)>	28.3
Completeness [%]	100.0
Redundancy	1.9
CC(1/2)	0.999	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	1.4M NaH2PO4/K2HPO4 pH 5.6