7JQH
Structure of wild type Glyoxylate/Hydroxypyruvate reductase A from Escherichia Coli in complex with phosphate and NADP+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-02-22 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 158.768, 158.768, 97.699 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.030 - 2.000 |
R-factor | 0.1536 |
Rwork | 0.153 |
R-free | 0.17370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kbo |
RMSD bond length | 0.014 |
RMSD bond angle | 1.280 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.030 | 2.070 |
High resolution limit [Å] | 1.999 | 1.999 |
Number of reflections | 49378 | 4836 |
<I/σ(I)> | 28.3 | |
Completeness [%] | 100.0 | |
Redundancy | 1.9 | |
CC(1/2) | 0.999 | 0.979 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 1.4M NaH2PO4/K2HPO4 pH 5.6 |