7JP8

Self-assembly of a 3D DNA crystal lattice (4x6 duplex version) containing the J10 immobile Holliday junction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	P 32
Unit cell lengths	67.524, 67.524, 53.314
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.762 - 3.103
R-factor	0.2305
Rwork	0.228
R-free	0.24730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vy6
RMSD bond length	0.005
RMSD bond angle	0.701
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.150
High resolution limit [Å]	3.100	8.400	3.100
Rmerge	0.106	0.083	0.575
Rmeas	0.116	0.091	0.635
Rpim	0.046	0.037	0.265
Number of reflections	4054	229	127
<I/σ(I)>	5.4
Completeness [%]	82.1	92.3	47.6
Redundancy	5.8	5.8	4.9
CC(1/2)	0.980	0.992	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.5 mL of 0.05 M TRIS pH 8.0 with 200 mM MgCl2 and 15% ethanol was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock