7JOI
Self-assembly of a 3D DNA crystal lattice (4x6 duplex version) containing the J30 immobile Holliday junction
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 32 |
Unit cell lengths | 68.025, 68.025, 53.298 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.013 - 3.148 |
R-factor | 0.2489 |
Rwork | 0.248 |
R-free | 0.26790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vy6 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.695 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
High resolution limit [Å] | 3.100 | 8.400 | 3.100 |
Rmerge | 0.131 | 0.099 | 0.563 |
Rmeas | 0.146 | 0.111 | 0.630 |
Rpim | 0.062 | 0.048 | 0.276 |
Number of reflections | 3235 | 249 | 55 |
<I/σ(I)> | 4.1 | ||
Completeness [%] | 63.9 | 98.4 | 25.3 |
Redundancy | 5.3 | 5.9 | 4 |
CC(1/2) | 1.000 | 0.995 | 0.841 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mL of 0.05 M Cacodylate pH 6.0 with 200 mM MgCl2 and 2.5 M KCl was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock |