7JOI
Self-assembly of a 3D DNA crystal lattice (4x6 duplex version) containing the J30 immobile Holliday junction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 |
| Unit cell lengths | 68.025, 68.025, 53.298 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.013 - 3.148 |
| R-factor | 0.2489 |
| Rwork | 0.248 |
| R-free | 0.26790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vy6 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.695 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 8.400 | 3.100 |
| Rmerge | 0.131 | 0.099 | 0.563 |
| Rmeas | 0.146 | 0.111 | 0.630 |
| Rpim | 0.062 | 0.048 | 0.276 |
| Number of reflections | 3235 | 249 | 55 |
| <I/σ(I)> | 4.1 | ||
| Completeness [%] | 63.9 | 98.4 | 25.3 |
| Redundancy | 5.3 | 5.9 | 4 |
| CC(1/2) | 1.000 | 0.995 | 0.841 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mL of 0.05 M Cacodylate pH 6.0 with 200 mM MgCl2 and 2.5 M KCl was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock |
