7JOH
Self-assembly of a 3D DNA crystal lattice (4x6 duplex version) containing the J31 immobile Holliday junction
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.92 |
Spacegroup name | P 32 |
Unit cell lengths | 68.759, 68.759, 55.211 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.380 - 4.195 |
R-factor | 0.2057 |
Rwork | 0.205 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vy6 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.621 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 4.270 |
High resolution limit [Å] | 4.195 | 11.360 | 4.200 |
Rmerge | 0.082 | 0.064 | 0.880 |
Rmeas | 0.089 | 0.071 | 0.944 |
Rpim | 0.033 | 0.029 | 0.336 |
Number of reflections | 2053 | 99 | 87 |
<I/σ(I)> | 4.9 | ||
Completeness [%] | 95.7 | 93.4 | 80.6 |
Redundancy | 7.7 | 6.4 | 7.3 |
CC(1/2) | 1.000 | 0.990 | 0.753 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mL of 0.05 M Cacodylate pH 6.0 with 200 mM, Ca(CH3COO)2, and 2.5 M NaCl was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock |