7JOF
Calcium-bound C2A Domain from Human Dysferlin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-02-05 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5 |
Spacegroup name | P 1 |
Unit cell lengths | 45.520, 54.780, 57.340 |
Unit cell angles | 116.80, 103.47, 92.92 |
Refinement procedure
Resolution | 9.986 - 2.000 |
R-factor | 0.2415 |
Rwork | 0.241 |
R-free | 0.24870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ihb |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 23.720 | 23.720 | 2.040 |
High resolution limit [Å] | 1.990 | 8.910 | 1.990 |
Rmerge | 0.061 | 0.042 | 0.155 |
Rmeas | 0.086 | 0.059 | 0.219 |
Rpim | 0.061 | 0.042 | 0.155 |
Total number of observations | 47404 | 540 | 2369 |
Number of reflections | 28309 | 288 | 1441 |
<I/σ(I)> | 9 | 18.9 | 4.5 |
Completeness [%] | 87.6 | 79.3 | 60.6 |
Redundancy | 1.7 | 1.9 | 1.6 |
CC(1/2) | 0.992 | 0.980 | 0.917 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 283.15 | 0.2 mM magnesium chloride hexahydrate, 0.1 M Tris hydrochloride, and 30% Polyethylene glycol 4,000 (commercial reagent kit from Hampton Research, HR2-130) at pH 8.5 |