7JO7

Crystal Structure of Human Scribble PDZ2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	80
Detector technology	CCD
Collection date	2017-06-09
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 1
Unit cell lengths	37.889, 41.688, 49.017
Unit cell angles	90.27, 91.96, 105.84

Refinement procedure

Resolution	31.600 - 2.440
R-factor	0.2205
Rwork	0.214
R-free	0.27360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDZ2-Vangl2 complex
RMSD bond length	0.005
RMSD bond angle	0.836
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.430	2.531
High resolution limit [Å]	2.440	2.440
Rmerge	0.120	0.650
Number of reflections	10516	1072
<I/σ(I)>	10.5	2.05
Completeness [%]	98.3	97.99
Redundancy	3.9	3.9
CC(1/2)	0.994	0.792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2M Potassium thiocyanate and 20% (w/v) PEG 3350