7JND
The structure of CBM32-1 and CBM32-2 domains from Clostridium perfringens ZmpB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-11 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91966 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.260, 81.970, 132.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.160 - 2.000 |
R-factor | 0.2369 |
Rwork | 0.235 |
R-free | 0.27920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7jnb |
RMSD bond length | 0.006 |
RMSD bond angle | 1.378 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.530 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.168 | 0.695 |
Rpim | 0.069 | 0.285 |
Number of reflections | 31552 | 4198 |
<I/σ(I)> | 6.4 | 2.5 |
Completeness [%] | 96.5 | 89.9 |
Redundancy | 6.5 | 6.6 |
CC(1/2) | 0.988 | 0.756 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.18 M calcium chloride, 20% PEG3350, 0.1 M HEPES |