7JMS
Structure of the Hazara virus OTU bound to ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-04-02 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.850, 55.595, 97.434 |
Unit cell angles | 90.00, 97.20, 90.00 |
Refinement procedure
Resolution | 41.590 - 2.780 |
R-factor | 0.2367 |
Rwork | 0.235 |
R-free | 0.27310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3prp 4hxd 5jze 6oar |
RMSD bond length | 0.006 |
RMSD bond angle | 0.950 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.830 |
High resolution limit [Å] | 2.780 | 2.780 |
Number of reflections | 22676 | 1993 |
<I/σ(I)> | 10.84 | 2.4 |
Completeness [%] | 96.9 | |
Redundancy | 4.7 | |
CC(1/2) | 0.990 | 0.641 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.3 M calcium chloride, 20% PEG 4000 |