7JMR
Crystal structure of the pea pathogenicity protein 2 from Madurella mycetomatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 98.853, 98.853, 62.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.740 - 1.670 |
| R-factor | 0.1782 |
| Rwork | 0.177 |
| R-free | 0.19550 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.029 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.740 | 1.690 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.070 | |
| Number of reflections | 78547 | 2702 |
| <I/σ(I)> | 40 | |
| Completeness [%] | 99.5 | 96 |
| Redundancy | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 15 % PEG 3350, 0.2 M Calcium Chloride, 0.1 M MES |
