7JM9
Sheep Connexin-50 at 2.5 angstroms reoslution, Lipid Class 2
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.5 |
| Software | RELION,EPU,Gctf |
| Symmetry | D6 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
