7JM2

Crystal structure of aminoglycoside resistance enzyme ApmA, complex with apramycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-20
Detector	PSI PILATUS 6M
Wavelength(s)	0.97860
Spacegroup name	P 21 21 21
Unit cell lengths	61.730, 107.536, 138.696
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.920 - 1.850
R-factor	0.1978
Rwork	0.197
R-free	0.23620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jm1
RMSD bond length	0.005
RMSD bond angle	0.779
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3448)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.880
High resolution limit [Å]	1.850	1.850
Rmerge	0.068	0.691
Rpim	0.041	0.459
Number of reflections	77441	3729
<I/σ(I)>	22.6	2.17
Completeness [%]	97.3
Redundancy	3.6
CC(1/2)		0.694

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M CaCl2, 20% PEG3350, 5 mM Apramycin cryoprotectant 25% Ethylene Glycol with 10 mM Apramycin