7JM1
Crystal structure of aminoglycoside resistance enzyme ApmA, complex with acetyl-CoA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97929 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.207, 77.233, 97.436 |
Unit cell angles | 90.00, 102.52, 90.00 |
Refinement procedure
Resolution | 37.840 - 2.310 |
R-factor | 0.1586 |
Rwork | 0.157 |
R-free | 0.19720 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.463 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15_3448) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.050 |
High resolution limit [Å] | 2.020 | 2.020 |
Rmerge | 0.046 | 0.296 |
Rpim | 0.030 | 0.214 |
Number of reflections | 42341 | 2929 |
<I/σ(I)> | 17.6 | 2.96 |
Completeness [%] | 96.9 | 92.4 |
Redundancy | 2.9 | |
CC(1/2) | 0.862 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M Citric Acid pH3.6, 30% PEG 200, 5mM Apramycin, 2mM AcCoA |