7JLH
Structure of Centromeric Satellite III Non-canonical Duplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 196 |
Detector technology | PIXEL |
Collection date | 2019-03-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.07110 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.522, 40.217, 55.379 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.540 - 1.570 |
R-factor | 0.224 |
Rwork | 0.221 |
R-free | 0.25510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 175d |
RMSD bond length | 0.017 |
RMSD bond angle | 1.668 |
Data reduction software | XDS (Jan 31, 2020) |
Data scaling software | XDS (Jan 31, 2020) |
Phasing software | PHASER (1.18_3845) |
Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.540 | 1.630 |
High resolution limit [Å] | 1.570 | 1.570 |
Number of reflections | 12855 | 643 |
<I/σ(I)> | 15.1 | 2.5 |
Completeness [%] | 99.7 | 99 |
Redundancy | 5.9 | 5.1 |
CC(1/2) | 0.998 | 0.991 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Tris-Acetate, potassium acetate, magnesium acetate, MES monohydrate, 2-Methyl-2,4-pentanediol |