7JKU

Crystal structure of a four-tetrad, parallel, and K+ stabilized Tetrahymena thermophila telomeric G-quadruplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	196
Detector technology	CCD
Collection date	2020-05-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 2 21 21
Unit cell lengths	32.042, 39.110, 59.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.600 - 1.970
R-factor	0.2484
Rwork	0.247
R-free	0.26150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	TEL26-1
RMSD bond length	0.006
RMSD bond angle	0.756
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.020	2.020
High resolution limit [Å]	1.970	1.970
Number of reflections	5471	381
<I/σ(I)>	7.7
Completeness [%]	97.9
Redundancy	6.3
CC(1/2)	0.994	0.343

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	sodium cacodylate, KCl, PEG, NaCl, ethylene glycol