7JKR
GTP-specific succinyl-CoA synthetase complexed with Mg-GMPPNP, phosphohistidine loop pointing towards nucleotide binding site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-07 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 99.328, 99.328, 134.029 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 86.020 - 2.640 |
R-factor | 0.2435 |
Rwork | 0.241 |
R-free | 0.29240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fp4 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3885) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.020 | 2.690 |
High resolution limit [Å] | 2.640 | 2.640 |
Rmerge | 0.123 | 2.191 |
Rpim | 0.052 | 0.938 |
Number of reflections | 23016 | 1140 |
<I/σ(I)> | 9.3 | 0.4 |
Completeness [%] | 100.0 | |
Redundancy | 6.4 | |
CC(1/2) | 0.997 | 0.298 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 1.0 M sodium citrate, Tris-HCl pH 7.3 |