7JJU

Crystal structure of en exoribonuclease-resistant RNA (xrRNA) from Potato leafroll virus (PLRV)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2019-09-13
Detector	RDI CMOS_8M
Wavelength(s)	0.9234
Spacegroup name	P 43 21 2
Unit cell lengths	72.190, 72.190, 137.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.720 - 2.604
R-factor	0.2261
Rwork	0.225
R-free	0.25340
Structure solution method	SAD
RMSD bond length	0.001
RMSD bond angle	0.441
Data reduction software	XDS (20191015)
Data scaling software	XSCALE (20191015)
Phasing software	PHENIX (1.13_2998)
Refinement software	PHENIX (dev_3885)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.720	2.697
High resolution limit [Å]	2.604	2.604
Rmerge	0.100	1.663
Rmeas	0.101	1.685
Rpim	0.016
Number of reflections	11689	1135
<I/σ(I)>	30.86	2.53
Completeness [%]	99.6	99.56
Redundancy	41.3	38
CC(1/2)	1.000	0.914

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1 microL RNA solution (5 mg/mL RNA in 30 mM HEPES pH 7.5, 20 mM MgCl2, 100 mM KCl) + 1 microL crystallization solution (10% 2-methyl-2,4-pentanediol, 40 mM Sodium Cacodylate (pH 6.0), 12 mM Spermine, 150 mM KCl, 100 mM Guanidinium HCl) over a reservoir of 500 microL of 30% 2-methyl-2,4-pentanediol and 100mM Guanidinium HCl.