7JJH

Crystal structure of the unliganded tandem bromodomain (BD1, BD2) of human TAF1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-15
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5417
Spacegroup name	C 2 2 21
Unit cell lengths	56.690, 199.720, 88.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.490 - 2.100
R-factor	0.1886
Rwork	0.188
R-free	0.21980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uv5
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.490	19.490	2.150
High resolution limit [Å]	2.100	9.390	2.100
Rmerge	0.074	0.026	0.414
Rmeas	0.076	0.027	0.429
Total number of observations	425655
Number of reflections	28879	333	2049
<I/σ(I)>	27.46	76.66	7.85
Completeness [%]	96.4	84.7	94.3
Redundancy	14.739	11.706	14.55
CC(1/2)	0.999	0.998	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.8 M Succinic Acid, pH 7.0