7JJF
Sarcin-ricin loop with modified residue.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | LIQUID ANODE |
Source details | Excillum MetalJet D2+ 70 kV |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-19 |
Detector | Bruker PHOTON III |
Wavelength(s) | 1.3418 |
Spacegroup name | P 43 |
Unit cell lengths | 29.340, 29.340, 75.661 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.360 - 1.200 |
R-factor | 0.184 |
Rwork | 0.177 |
R-free | 0.21400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dvz |
RMSD bond length | 0.007 |
RMSD bond angle | 1.342 |
Data reduction software | PROTEUM PLUS |
Data scaling software | PROTEUM PLUS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.360 | 1.240 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.089 | |
<I/σ(I)> | 23.47 | |
Completeness [%] | 94.1 | |
Redundancy | 17 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | RNA was dissolved in 1 mM Na2 EDTA (pH 8.0) and 10 mM Tris-HCl (pH 8.0) to attain 350 uM final RNA concentration. The RNA sample was annealed by heating at 65 C for 2 min and slowly cooling to room temperature. Crystallization set-ups were made by mixing 4 uL of RNA solution with 2 uL of a crystallization buffer composed of 3.0 M ammonium sulfate, 10 mM magnesium chloride, 10 mM manganese chloride, and 50 mM potassium 3-(N-morpholino) propanesulfonic acid (MOPS), pH 7.0 at 18 C. Crystals appear in about two weeks time. |