7JIX

Murine antibody that engages the influenza hemagglutinin receptor binding site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.999935
Spacegroup name	P 21 3
Unit cell lengths	198.507, 198.507, 198.507
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.150 - 3.901
R-factor	0.217
Rwork	0.215
R-free	0.25490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ugy
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.150	4.040
High resolution limit [Å]	3.900	3.901
Number of reflections	25174	2324
<I/σ(I)>	8.3
Completeness [%]	97.7
Redundancy	1.1
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% PEG 400, CHES pH 9.0, 100mM MgSO4