7JI4
Universal stress protein (USP) domain of KdpD histidine kinase in complex with second messenger c-di-AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2018-07-02 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97741 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 54.411, 54.411, 96.526 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.400 - 2.300 |
R-factor | 0.2118 |
Rwork | 0.208 |
R-free | 0.24900 |
Structure solution method | SAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.17.1-3660-000) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
Rmerge | 0.112 | 0.088 | 0.444 |
Rmeas | 0.115 | 0.091 | 0.454 |
Rpim | 0.023 | 0.019 | 0.092 |
Total number of observations | 164630 | ||
Number of reflections | 6957 | 423 | 331 |
<I/σ(I)> | 8.3 | ||
Completeness [%] | 100.0 | 100 | 99.1 |
Redundancy | 23.7 | 20.8 | 21.8 |
CC(1/2) | 0.997 | 0.982 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.11 M lithium sulfate, 0.1 M Tris-HCl, pH 8.5, 21% PEG3350 |