7JI0
CryoEM structure of Streptococcus thermophilus SHP pheromone receptor Rgg3 in complex with SHP3
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TALOS ARCTICA |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 2.7 |
Energy Filter | GIF Bioquantum |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.95 |
Number of Particle | 389743 |
Software | cryoSPARC,EPU,UCSF Chimera,PHENIX |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |