7JHU
Self-assembly of a 3D DNA crystal lattice (4x6 junction version) containing the J33 immobile Holliday junction with R3 symmetry
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.92 |
Spacegroup name | H 3 |
Unit cell lengths | 113.869, 113.869, 50.807 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.053 - 3.152 |
R-factor | 0.2451 |
Rwork | 0.244 |
R-free | 0.27270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xna |
RMSD bond length | 0.005 |
RMSD bond angle | 0.768 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.200 |
High resolution limit [Å] | 3.150 | 8.540 | 3.150 |
Rmerge | 0.136 | 0.131 | 0.423 |
Rmeas | 0.149 | 0.144 | 0.472 |
Rpim | 0.058 | 0.057 | 0.201 |
Number of reflections | 3676 | 212 | 78 |
<I/σ(I)> | 6 | ||
Completeness [%] | 86.4 | 100 | 35.3 |
Redundancy | 6.9 | 8.9 | 3.7 |
CC(1/2) | 0.905 | 0.891 | 0.867 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mL of 0.05 M Cacodylate pH 6.5 with 18 mM MgCl2, 2.25 mM spermine, and 9% Isopropanol was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock |