7JHT
Self-assembly of a 3D DNA crystal lattice (4x6 junction version) containing the J31 immobile Holliday junction with R3 symmetry
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | H 3 |
Unit cell lengths | 116.078, 116.078, 49.430 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.358 - 3.190 |
R-factor | 0.2236 |
Rwork | 0.222 |
R-free | 0.25840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xna |
RMSD bond length | 0.005 |
RMSD bond angle | 0.679 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.200 |
High resolution limit [Å] | 3.150 | 8.540 | 3.150 |
Rmerge | 0.116 | 0.085 | 0.451 |
Rmeas | 0.123 | 0.090 | 0.490 |
Rpim | 0.040 | 0.029 | 0.187 |
Number of reflections | 3347 | 204 | 71 |
<I/σ(I)> | 5.6 | ||
Completeness [%] | 81.1 | 99 | 30.9 |
Redundancy | 8.4 | 10.3 | 5.2 |
CC(1/2) | 0.948 | 0.998 | 0.899 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mL of 0.05 M Cacodylate pH 6.5 with 2.5 mM spermine, 18 mM CaCl2, and 9% 2-propanol was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock |