7JHR
Self-assembly of a 3D DNA crystal lattice (4x6 junction version) containing the J4 immobile Holliday junction with R3 symmetry
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | H 3 |
Unit cell lengths | 115.150, 115.150, 48.657 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.729 - 3.158 |
R-factor | 0.2491 |
Rwork | 0.245 |
R-free | 0.27750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xna |
RMSD bond length | 0.004 |
RMSD bond angle | 0.635 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.200 |
High resolution limit [Å] | 3.150 | 8.540 | 3.150 |
Rmerge | 0.061 | 0.042 | 0.308 |
Rmeas | 0.064 | 0.045 | 0.328 |
Rpim | 0.020 | 0.014 | 0.109 |
Number of reflections | 3297 | 205 | 62 |
<I/σ(I)> | 6.3 | ||
Completeness [%] | 79.4 | 99.5 | 26.6 |
Redundancy | 8.7 | 10.6 | 6.7 |
CC(1/2) | 1.000 | 1.000 | 0.967 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mL of 0.05 M Cacodylate pH 7.0 with 20 mM MgCl2, 1.0 mM spermine, 1.0 mM CoH18N6, and 15% Ethanol was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock |