7JHC
Self-assembly of a 3D DNA crystal lattice (4x6 junction version) containing the J26 immobile Holliday junction
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-12-15 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1 |
Spacegroup name | P 32 |
Unit cell lengths | 68.172, 68.172, 55.083 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.086 - 3.110 |
R-factor | 0.265 |
Rwork | 0.264 |
R-free | 0.27740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xna |
RMSD bond length | 0.005 |
RMSD bond angle | 0.678 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
High resolution limit [Å] | 3.100 | 8.400 | 3.100 |
Rmerge | 0.097 | 0.036 | 0.526 |
Rmeas | 0.105 | 0.038 | 0.569 |
Rpim | 0.038 | 0.014 | 0.212 |
Number of reflections | 4614 | 254 | 133 |
<I/σ(I)> | 8 | ||
Completeness [%] | 89.7 | 98.1 | 57.8 |
Redundancy | 7.1 | 7.4 | 5.7 |
CC(1/2) | 0.994 | 0.999 | 0.962 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mL of 0.05 M TRIS pH 7.5 with 200 mM MgCl2 and 2.5 M NaCl was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock |