7JH3
Crystal structure of 4-aminobutyrate aminotransferase PuuE from Escherichia coli in complex with PLP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-09-29 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 198.641, 78.839, 164.976 |
Unit cell angles | 90.00, 112.82, 90.00 |
Refinement procedure
Resolution | 24.880 - 2.680 |
R-factor | 0.1794 |
Rwork | 0.178 |
R-free | 0.21130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6wj8 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.712 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.730 |
High resolution limit [Å] | 2.680 | 2.680 |
Rpim | 0.051 | 0.235 |
Number of reflections | 67076 | 3334 |
<I/σ(I)> | 12.1 | 2 |
Completeness [%] | 98.7 | |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 0.1M di-Na Tartrate, 0.1M Tris pH8.5, 20%PEG3350, 2.2mM PLP, 1%Et.Glycol |