7JGZ

Protocadherin gammaC4 EC1-4 crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979180
Spacegroup name	I 21 21 21
Unit cell lengths	75.220, 115.680, 194.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.500 - 3.510
R-factor	0.2257
Rwork	0.223
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zpo
RMSD bond length	0.004
RMSD bond angle	0.889
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.840
High resolution limit [Å]	3.500	3.500
Rmerge	0.215	0.830
Rmeas	0.232	0.896
Rpim	0.086	0.335
Number of reflections	7545	340
<I/σ(I)>	8.3	2.9
Completeness [%]	67.6	12.5
Redundancy	7.1	7.1
CC(1/2)	0.997	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	10% (w/v) PEG8000, 20% ethylene glycol, 10% Morpheus Amino Acids (Molecular Dimensions), and 0.1 M Morpheus Buffer System 2 (Hepes/MOPS buffer) pH 7.5