7JFY

GAS41 YEATS domain in complex with 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-13
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97857
Spacegroup name	C 1 2 1
Unit cell lengths	117.266, 112.503, 63.222
Unit cell angles	90.00, 116.74, 90.00

Refinement procedure

Resolution	39.849 - 2.101
R-factor	0.210363055247
Rwork	0.208
R-free	0.26422
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vna
RMSD bond length	0.002
RMSD bond angle	0.509
Data scaling software	HKL-2000 (1.11.1_2575)
Phasing software	MOLREP
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.850	2.176
High resolution limit [Å]	2.100	2.101
Rmerge	0.091	0.480
Rmeas	0.106	0.558
Rpim	0.054	0.283
Number of reflections	42637	4184
<I/σ(I)>	11.74	2.49
Completeness [%]	98.1
Redundancy	3.9
CC(1/2)	0.994	0.809

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	277	0.1 M Tris, pH 7.3, 0.2 M magnesium chloride, 2.2 M sodium chloride