7JFY
GAS41 YEATS domain in complex with 5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-10-13 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 117.266, 112.503, 63.222 |
Unit cell angles | 90.00, 116.74, 90.00 |
Refinement procedure
Resolution | 39.849 - 2.101 |
R-factor | 0.210363055247 |
Rwork | 0.208 |
R-free | 0.26422 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vna |
RMSD bond length | 0.002 |
RMSD bond angle | 0.509 |
Data scaling software | HKL-2000 (1.11.1_2575) |
Phasing software | MOLREP |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.850 | 2.176 |
High resolution limit [Å] | 2.100 | 2.101 |
Rmerge | 0.091 | 0.480 |
Rmeas | 0.106 | 0.558 |
Rpim | 0.054 | 0.283 |
Number of reflections | 42637 | 4184 |
<I/σ(I)> | 11.74 | 2.49 |
Completeness [%] | 98.1 | |
Redundancy | 3.9 | |
CC(1/2) | 0.994 | 0.809 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 277 | 0.1 M Tris, pH 7.3, 0.2 M magnesium chloride, 2.2 M sodium chloride |