7JFW
Self-assembly of a 3D DNA crystal lattice (4x6 junction version) containing the J10 immobile Holliday junction
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 32 |
Unit cell lengths | 67.739, 67.739, 53.477 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.870 - 3.012 |
R-factor | 0.1935 |
Rwork | 0.192 |
R-free | 0.21730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xna |
RMSD bond length | 0.006 |
RMSD bond angle | 0.809 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
High resolution limit [Å] | 3.012 | 8.400 | 3.100 |
Rmerge | 0.106 | 0.083 | 0.575 |
Rmeas | 0.116 | 0.091 | 0.635 |
Rpim | 0.046 | 0.037 | 0.265 |
Number of reflections | 4200 | 229 | 127 |
<I/σ(I)> | 5.4 | ||
Completeness [%] | 82.1 | 92.3 | 47.6 |
Redundancy | 5.8 | 5.8 | 4.9 |
CC(1/2) | 0.980 | 0.992 | 0.892 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mL of 0.05 M TRIS pH 8.0 with 200 mM MgCl2, and 15% Ethanol was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock |