7GWR
Crystal Structure of B-cell lymphoma 6 protein BTB domain in complex with ligand 6 at 14.76 MGy X-ray dose.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-31 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 67.870, 67.870, 167.370 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.090 - 1.900 |
R-factor | 0.184 |
Rwork | 0.183 |
R-free | 0.20300 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.010 |
RMSD bond angle | 0.950 |
Data scaling software | Aimless (0.5.32) |
Phasing software | BUSTER (2.10.3) |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.090 | 34.090 | 1.940 |
High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
Rmerge | 0.104 | 0.045 | 1.481 |
Rmeas | 0.110 | 0.048 | 1.571 |
Rpim | 0.035 | 0.017 | 0.507 |
Total number of observations | 168872 | 1502 | 10924 |
Number of reflections | 18295 | 208 | 1159 |
<I/σ(I)> | 9.8 | 30.3 | 1.4 |
Completeness [%] | 98.3 | 94.1 | 99.4 |
Redundancy | 9.2 | 7.2 | 9.4 |
CC(1/2) | 0.997 | 0.998 | 0.625 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 1.0 M K2HPO4, 0.7 M NaH2PO4, 75 mM sodium acetate pH 4.5, 2% DMSO |