7GR1

Crystal Structure of PAS-GAF domain of the phytochrome from Deinococcus radiodurans at femto and picosecond timescales (109 fs)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	298
Detector technology	CCD
Collection date	2021-06-24
Detector	MPCCD
Wavelength(s)	1.7712
Spacegroup name	P 21 21 21
Unit cell lengths	54.950, 116.500, 117.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.120
R-factor	0.1897
Rwork	0.190
R-free	0.18890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.384
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	33.070
High resolution limit [Å]	2.120
Number of reflections	41974
<I/σ(I)>	8.66
Completeness [%]	100.0
Redundancy	136
CC(1/2)	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	4.95	298	60 mM sodium acetate, 3.3% PEG400, 1 mM DTT, 30% MPD