7GQW

Crystal Structure of PAS-GAF domain of the phytochrome from Deinococcus radiodurans at femto and picosecond timescales (-40 fs)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	298
Detector technology	CCD
Collection date	2021-06-24
Detector	MPCCD
Wavelength(s)	1.7712
Spacegroup name	P 21 21 21
Unit cell lengths	54.950, 116.500, 117.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.120
R-factor	0.2135
Rwork	0.213
R-free	0.22260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.393
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	33.070
High resolution limit [Å]	2.120
Number of reflections	43737
<I/σ(I)>	6.93
Completeness [%]	100.0
Redundancy	64.33
CC(1/2)	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	4.95	298	60 mM sodium acetate, 3.3% PEG400, 1 mM DTT, 30% MPD