7GAM
PanDDA analysis group deposition -- Crystal Structure of MAP1LC3B in complex with Z373768900
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 |
| Unit cell lengths | 60.681, 60.681, 35.614 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.680 - 1.750 |
| R-factor | 0.1981 |
| Rwork | 0.197 |
| R-free | 0.23440 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8q53 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.626 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.670 | 60.670 | 1.780 |
| High resolution limit [Å] | 1.750 | 9.100 | 1.750 |
| Rmerge | 0.068 | 0.062 | 2.668 |
| Rmeas | 0.071 | 0.066 | 2.780 |
| Rpim | 0.019 | 0.020 | 0.774 |
| Total number of observations | 180563 | 1150 | 9230 |
| Number of reflections | 13277 | 111 | 723 |
| <I/σ(I)> | 14.3 | 51.5 | 0.4 |
| Completeness [%] | 99.9 | 99.7 | 98.3 |
| Redundancy | 13.6 | 10.4 | 12.8 |
| CC(1/2) | 0.999 | 0.998 | 0.408 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 293 | 36% PEG 8000, 0.1M acetate pH 4.7 |
