7GAK
PanDDA analysis group deposition -- Crystal Structure of MAP1LC3B in complex with Z287121492
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 |
| Unit cell lengths | 60.807, 60.807, 35.097 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.420 - 1.770 |
| R-factor | 0.2024 |
| Rwork | 0.200 |
| R-free | 0.24970 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8q53 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.619 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.400 | 30.400 | 1.810 |
| High resolution limit [Å] | 1.770 | 9.040 | 1.770 |
| Rmerge | 0.104 | 0.053 | 6.109 |
| Rmeas | 0.109 | 0.056 | 6.361 |
| Rpim | 0.030 | 0.017 | 1.764 |
| Total number of observations | 173163 | 1171 | 9502 |
| Number of reflections | 12673 | 110 | 736 |
| <I/σ(I)> | 13.6 | 69.1 | 0.3 |
| Completeness [%] | 100.0 | 98.6 | 99.4 |
| Redundancy | 13.7 | 10.6 | 12.9 |
| CC(1/2) | 0.997 | 0.998 | 0.382 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 293 | 36% PEG 8000, 0.1M acetate pH 4.7 |
