7GAH
PanDDA analysis group deposition -- Crystal Structure of MAP1LC3B in complex with Z2033637875
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 |
| Unit cell lengths | 61.200, 61.200, 35.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.580 - 1.900 |
| R-factor | 0.2008 |
| Rwork | 0.199 |
| R-free | 0.25410 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8q53 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.628 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.600 | 30.600 | 1.950 |
| High resolution limit [Å] | 1.900 | 8.500 | 1.900 |
| Rmerge | 0.127 | 0.054 | 2.878 |
| Rmeas | 0.133 | 0.057 | 2.985 |
| Rpim | 0.038 | 0.017 | 0.787 |
| Total number of observations | 138579 | 1261 | 11039 |
| Number of reflections | 10488 | 124 | 771 |
| <I/σ(I)> | 15 | 37 | 1 |
| Completeness [%] | 100.0 | 98.1 | 100 |
| Redundancy | 13.2 | 10.2 | 14.3 |
| CC(1/2) | 0.997 | 0.998 | 0.365 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 293 | 36% PEG 8000, 0.1M acetate pH 4.7 |
