7G9I
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with PCM-0102253-001
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | DIAMOND |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-03-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.92124 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 70.675, 70.675, 195.449 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.990 - 2.202 |
R-factor | 0.2153 |
Rwork | 0.212 |
R-free | 0.27380 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 8bnt |
RMSD bond length | 0.008 |
RMSD bond angle | 0.930 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.9) |
Phasing software | REFMAC |
Refinement software | BUSTER (2.10.4 (17-FEB-2023)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 97.720 | 97.720 | 2.320 |
High resolution limit [Å] | 2.200 | 6.960 | 2.200 |
Rmerge | 0.683 | 0.087 | 9.454 |
Rmeas | 0.705 | 0.090 | 9.739 |
Rpim | 0.171 | 0.024 | 2.319 |
Total number of observations | 411384 | 12708 | 60663 |
Number of reflections | 25765 | ||
<I/σ(I)> | 5.5 | 18.5 | 0.9 |
Completeness [%] | 99.3 | 99.7 | 98.7 |
Redundancy | 16 | 13.2 | 16.6 |
CC(1/2) | 0.993 | 0.998 | 0.558 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |